Search results for "Specific enzyme"
showing 2 items of 2 documents
Development of a sensitive and specific enzyme-linked immunosorbent assay for the determination of fludioxonil residues in fruit juices
2014
Fludioxonil is a fungicide with a singular mode of action that is widely employed in the treatment of fruit crops. A competitive enzyme-linked immunosorbent assay (cELISA) has been developed and validated for the determination of residues of this fungicide in fruit juices. A collection of monoclonal antibodies (mAbs) against the fungicide fludioxonil has been produced and characterized using direct and indirect cELISAs. The high affinity achieved (IC50lower than 0.5 μg L-1) considerably improved that obtained with previous polyclonal antibodies. The proposed cELISA was applied to the analysis of fludioxonil residues in apple juice and red grape must samples with a limit of quantification of…
Soil profile dismantlement by land levelling and deep tillage damages soil functioning but not quality
2016
We investigated the effects of land levelling followed by deep tillage, thus inducing a drastic dismantlement of soil profile, on both soil functioning and quality by monitoring various bioindicators (microbial biomass and community structure, basal respiration, enzyme activities) expressed on either whole soil and TOC mass units, respectively. As expected, in disturbed soils all measured properties had much higher coefficients of variation (CVs), regardless of either whole soil or TOC mass basis, due to the induced spatial variability. The amount of total organic C in the first cubic meter of soil profile was of one order of magnitude greater in undisturbed soils compared to disturbed ones…